Book GeneXpert Test in Bareilly

The GeneXpert test is a rapid molecular diagnostic test that detects the genetic material of Mycobacterium tuberculosis, the bacterium responsible for tuberculosis, and simultaneously identifies resistance to rifampicin, one of the most critical first-line anti-TB drugs. The test uses a technology called real-time polymerase chain reaction, commonly known as RT-PCR, to amplify and detect specific DNA sequences unique to the TB bacterium directly from a patient sample within approximately two hours, compared to the weeks required for conventional TB culture methods. Developed and endorsed by the World Health Organization, the GeneXpert MTB/RIF assay has transformed TB diagnosis globally and holds particular significance in India, which carries the highest TB burden of any country in the world, accounting for approximately 27 percent of all global TB cases. The test is a cornerstone of India's National TB Elimination Programme and is widely used across government and private healthcare facilities to accelerate diagnosis, reduce transmission, and identify drug-resistant TB cases that require specialised treatment regimens. Unlike sputum smear microscopy, which misses a significant proportion of TB cases, GeneXpert is highly sensitive and can detect TB even in paucibacillary samples where bacterial load is low. The test is most commonly performed on a sputum sample, though it can also be applied to other specimens including bronchoalveolar lavage, cerebrospinal fluid, lymph node aspirates, and pleural fluid depending on the suspected site of infection.

Doctors prescribe a GeneXpert test in the following situations: Diagnosing pulmonary tuberculosis in patients with chronic cough lasting more than two weeks, haemoptysis, unexplained weight loss, night sweats, and low-grade fever where rapid molecular confirmation of TB is clinically essential for initiating treatment without delay and preventing further transmission in the community. Detecting rifampicin resistance in newly diagnosed or previously treated TB patients where identification of drug resistance at the point of diagnosis is critical for immediately directing patients toward appropriate drug-resistant TB treatment regimens rather than ineffective standard therapy. Evaluating HIV-positive patients with suspected TB where the risk of TB is significantly higher, clinical presentation is frequently atypical, sputum smear sensitivity is reduced due to low bacterial loads, and rapid molecular diagnosis is essential for early treatment initiation given the high mortality of untreated TB-HIV coinfection. Investigating extrapulmonary tuberculosis including TB meningitis, TB lymphadenitis, pleural TB, and spinal TB where GeneXpert applied to appropriate non-sputum specimens provides rapid molecular confirmation in clinical situations where conventional diagnosis is slow and delayed treatment carries severe consequences. Assessing treatment failure or relapse in patients who have previously completed TB therapy and present with recurrent symptoms, where GeneXpert rapidly identifies whether viable TB bacteria are present and whether rifampicin resistance has emerged during prior treatment. Screening high-risk contacts of confirmed drug-resistant TB cases where early molecular testing enables rapid identification of secondary cases and prompt initiation of appropriate treatment before disease progression and further transmission occur.

The GeneXpert test detects the presence or absence of Mycobacterium tuberculosis DNA and assesses rifampicin resistance through molecular analysis of the rpoB gene. Unlike conventional blood tests, results are reported qualitatively rather than as a numerical value. MTB Not Detected A not detected result indicates that Mycobacterium tuberculosis DNA was not identified in the sample tested. This effectively rules out TB in the tested specimen with high confidence, though clinical judgement remains important as a single negative result does not entirely exclude TB in patients with strong clinical and radiological suspicion, and repeat testing or alternative specimen collection may be warranted. MTB Detected, Rifampicin Sensitive This result confirms the presence of TB bacteria in the sample and indicates that the strain is sensitive to rifampicin, meaning standard first-line anti-TB treatment with the RNTCP or NTEP regimen is appropriate and expected to be effective with good treatment adherence. MTB Detected, Rifampicin Resistant This result confirms TB and identifies resistance to rifampicin, which is a strong proxy marker for multidrug-resistant tuberculosis. This finding requires immediate referral to a drug-resistant TB treatment centre, initiation of a second-line drug regimen, contact tracing, and notification to the national TB programme as per NTEP guidelines.

The GeneXpert test is most commonly performed on a sputum sample, and the quality of sputum collection is critical for obtaining an accurate result. Patients should be instructed to provide a deep cough sputum specimen collected early in the morning before eating or drinking, as early morning sputum has the highest bacterial concentration and yields the best diagnostic sensitivity. Before providing the sputum sample, rinse your mouth thoroughly with plain water but do not use mouthwash or toothpaste immediately before collection, as antiseptic agents can inhibit the PCR reaction and reduce test sensitivity. Collect the specimen in the sterile container provided by the laboratory, ensuring it contains actual sputum from the lower respiratory tract rather than saliva from the mouth. No fasting or medication restrictions are required specifically for this test. However, if you are already taking anti-TB medications, inform your doctor before the test as prior antibiotic exposure can reduce bacterial load in the sample and may affect the sensitivity of detection in some cases. Sample transport to the laboratory should be prompt, and if any delay is anticipated, the sample should be refrigerated at 2 to 8 degrees Celsius to preserve specimen integrity.

If you are booking through the SecondMedic platform the GeneXpert test price in Bareilly can cost you around Rs. 3126. You may also consider booking a comprehensive TB diagnostic panel that includes GeneXpert alongside chest X-ray, ESR, and complete blood count for a thorough tuberculosis workup at a bundled price on SecondMedic.

SecondMedic offers convenient home sample collection for the GeneXpert test in Bareilly, making it easy to get tested without visiting a diagnostic centre. Home collection is available free of charge for orders above Rs. 300. A trained phlebotomist will visit your preferred address between 7 AM and 10 PM, seven days a week, including Sundays and public holidays. Your sample is processed at NABL-accredited partner laboratories, and your report is delivered within 24 hours directly to your WhatsApp and email.