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Quick Testosterone Test Overview

Also Known As Serum Testosterone Test, Total Testosterone Test
Sample Type Blood (Venous)
Fasting Required Not strictly required; morning collection between 7 AM and 10 AM recommended
Report Delivery Within 24 hours
Age Group All age groups
Gender All
Test Type Chemiluminescent Immunoassay
Units ng/dL or nmol/L
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The Testosterone test is a blood test that measures the level of testosterone, the primary male sex hormone produced mainly by the testes in men and in smaller amounts by the ovaries and adrenal glands in women. Testosterone plays a fundamental role in male development including muscle mass, bone density, facial and body hair, sperm production, libido, and overall energy levels. In women, testosterone contributes to bone strength, sexual drive, and hormonal balance. Testosterone deficiency in men, known as hypogonadism, is increasingly recognised in India and is associated with fatigue, reduced libido, erectile dysfunction, loss of muscle mass, weight gain, depression, and poor concentration. Conversely, elevated testosterone in women is a hallmark of Polycystic Ovary Syndrome (PCOS), one of the most prevalent hormonal disorders among Indian women of reproductive age. The Testosterone test is therefore clinically relevant across both sexes and multiple age groups. It is measured as total testosterone, and in some cases free testosterone is also assessed. The test involves a simple blood draw completed in under five minutes, ideally collected in the morning when testosterone levels are at their peak.
2

Doctors prescribe a Testosterone test in the following situations: Investigating symptoms of testosterone deficiency in men including persistent fatigue, low libido, erectile dysfunction, reduced muscle strength, increased body fat, mood disturbances, and poor concentration that do not resolve with general measures. Diagnosing hypogonadism in men where low testosterone production may be due to primary testicular failure or secondary causes involving the pituitary or hypothalamus. Evaluating delayed puberty in adolescent boys where testosterone levels help determine whether pubertal development is progressing appropriately for age. Investigating infertility in men as testosterone is essential for sperm production and low levels are frequently associated with reduced sperm count and poor semen quality. Diagnosing PCOS in women presenting with irregular periods, excess facial or body hair, acne, scalp hair thinning, and difficulty conceiving, where elevated testosterone is a key diagnostic marker. Evaluating adrenal disorders including congenital adrenal hyperplasia and adrenal tumours where abnormal testosterone production originates from the adrenal glands rather than the gonads. Monitoring testosterone replacement therapy in men being treated for hypogonadism to ensure levels are maintained within the therapeutic range safely and effectively.
3

The Testosterone test measures the concentration of total testosterone in the blood, reported in nanograms per decilitre (ng/dL) or nanomoles per litre (nmol/L). Normal Testosterone Range in India The standard reference ranges used across most Indian diagnostic laboratories are as follows. For adult men, a normal total testosterone level is between 300 and 1,000 ng/dL. For adult women, a normal total testosterone level is between 15 and 70 ng/dL. Interpreting Testosterone Results A testosterone level below 300 ng/dL in men confirms hypogonadism and typically warrants further evaluation to identify the underlying cause before considering testosterone replacement therapy. A testosterone level between 300 and 400 ng/dL in men with symptoms of deficiency is considered borderline low and is interpreted alongside clinical symptoms, free testosterone, and other hormonal markers such as LH and FSH. A testosterone level above 70 ng/dL in women, particularly when accompanied by symptoms such as irregular periods, hirsutism, and acne, raises concern for PCOS, adrenal hyperplasia, or an androgen-secreting tumour and warrants further hormonal workup.
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The blood sample for testosterone should ideally be collected between 7 AM and 10 AM as testosterone levels follow a diurnal rhythm and are highest in the morning. A sample collected later in the day may show falsely lower values. Our team confirms preparation requirements at the time of booking. Fasting is not strictly required for testosterone testing alone. However if testosterone is part of a broader hormonal or metabolic panel that includes fasting parameters, fast as advised by your doctor. Inform the phlebotomist about all medications you are currently taking. Anabolic steroids, corticosteroids, opioids, and hormonal therapies directly affect testosterone levels and must be disclosed for accurate interpretation. Avoid alcohol consumption for at least 24 hours before the test as alcohol suppresses testosterone production and can produce a misleadingly low result. Stay normally hydrated before sample collection.
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If you are booking through the SecondMedic platform the Testosterone test price starts at approximately Rs. 670. The exact price will be confirmed at the time of booking through SecondMedic. If your doctor has prescribed multiple tests alongside Testosterone, SecondMedic health packages include Testosterone as part of a broader hormonal or reproductive health panel at a significantly lower combined price.
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SecondMedic provides home sample collection for Testosterone test across all major areas. You do not need to visit a lab or collection centre. A certified and trained phlebotomist comes to your home or workplace at your chosen time, collects the sample using sterile single-use equipment, and ensures it is transported to the NABL-accredited lab within the required time window for accurate processing. Please note that SecondMedic provides free home sample collection on all tests priced above Rs. 300. Our team will check your pincode and confirm if your address falls under our free sample collection eligibility criteria, which depends upon the lab location and phlebotomist availability. Home collection is available between 7 AM and 10 PM, seven days a week, including Sundays and public holidays. For testosterone testing, booking the earliest available morning slot between 7 AM and 10 AM is strongly recommended for the most accurate result. Enter your pincode on the booking page or call our helpline to confirm availability at your address.

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People Also Ask

The Testosterone test is prescribed to diagnose hypogonadism in men with fatigue, low libido, and erectile dysfunction, to evaluate PCOS in women with irregular periods and excess hair growth, and to investigate delayed puberty and male infertility. It is also used to monitor testosterone replacement therapy and evaluate adrenal hormone disorders.

The blood sample should ideally be collected between 7 AM and 10 AM as testosterone follows a diurnal rhythm and is highest in the morning. A sample collected later in the day may show falsely lower values and lead to an inaccurate interpretation of testosterone status.

Fasting is not strictly required for testosterone testing alone. However, if testosterone is part of a broader hormonal or metabolic panel that includes fasting parameters, fast as advised by your doctor. Avoiding alcohol for at least 24 hours before the test is recommended regardless.

Normal total testosterone levels for adult men in India are between 300 and 1,000 ng/dL. Levels below 300 ng/dL confirm hypogonadism and typically warrant further evaluation of pituitary and testicular function before considering testosterone replacement therapy.

A testosterone level below 300 ng/dL in men indicates hypogonadism and may explain symptoms of fatigue, low libido, erectile dysfunction, and reduced muscle mass. Further evaluation of LH, FSH, and prolactin is typically done alongside to identify whether the cause is primary testicular failure or a secondary pituitary or hypothalamic problem.

An elevated testosterone above 70 ng/dL in women is most commonly associated with PCOS or adrenal disorders. When accompanied by symptoms such as irregular periods, hirsutism, and acne, it warrants a complete hormonal workup including LH, FSH, DHEAS, and pelvic ultrasound.

Yes, anabolic steroids, corticosteroids, opioids, and hormonal therapies directly affect testosterone levels. Alcohol consumption also suppresses testosterone production and should be avoided for at least 24 hours before the test. Always inform your doctor and phlebotomist about all medications and supplements you are currently taking.

Testosterone follows a diurnal rhythm, meaning levels are naturally highest between 7 AM and 10 AM and gradually decline through the day. A sample collected in the afternoon or evening may show values that are 20 to 30% lower than the true morning peak, which can lead to a false diagnosis of hypogonadism in men with borderline levels.

The Testosterone test report is delivered within 24 hours of sample collection directly on WhatsApp and email. No physical visit to any collection centre is required to collect the report.

Content Reviewed By

Reviewer
Reviewed by:

Dr. Kovid Pandey

MBBS, General Physician

Last Reviewed: 10th Mar 2026

References

1
Bhasin S et al.: Testosterone Therapy in Men with Hypogonadism: An Endocrine Society Clinical Practice Guideline, Journal of Clinical Endocrinology and Metabolism, 2018 — pubmed.ncbi.nlm.nih.gov
2
Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group: Revised Criteria for Polycystic Ovary Syndrome, Human Reproduction, 2004 — pubmed.ncbi.nlm.nih.gov
3
Dandona P, Rosenberg MT: A Practical Guide to Male Hypogonadism in the Primary Care Setting, International Journal of Clinical Practice, 2010 — pubmed.ncbi.nlm.nih.gov
4
Unnikrishnan AG et al.: Polycystic Ovary Syndrome in Indian Adolescents, Indian Journal of Endocrinology and Metabolism, 2010 — pubmed.ncbi.nlm.nih.gov
5
Vermeulen A, Verdonck L, Kaufman JM: A Critical Evaluation of Simple Methods for the Estimation of Free Testosterone in Serum, Journal of Clinical Endocrinology and Metabolism, 1999 — pubmed.ncbi.nlm.nih.gov

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